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Chimerism Studies


DNA/RNA
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Molecular Haematology


SEX MATCHED TRANSPLANT SPECIMENS


Specimen Collection

Note: All samples should be forwarded to LabPlus at room temperature within 24hours.


CPD4 mL CPD Blood (Preferred)
CPD4 mL CPD Bone Marrow (Preferred)
EDTA4 mL EDTA Blood
EDTA4 mL EDTA Bone Marrow
Micro-EDTA0.5 mL Paediatric Micro-EDTA Blood
Micro-EDTA0.5 mL Paediatric Micro-EDTA Bone Marrow
Turnaround Time: Within 2 weeks, 4 days
Diagnostic Use and Interpretation

This test uses DNA profiling to determine the contribution of donor or recipient DNA within a blood or bone marrow sample (although conceivably any sample from which DNA may be extracted could be used in this test, including blood enriched for Lymphoid (CD3+) cells (see link below)) after a patient has undergone a bone marrow transplant.  The DNA profiling is performed using a panel of usually 16 different loci. A percentage contribution is calculated for each loci and the average is taken to give the percentage donor and/or recipient content.

DNA Chimerism studies will be required following a sex-matched bone marrow transplant. Please submit a second CPD tube with the Donor Storage specimen, if this service is clinically indicated.
If a bone marrow transplant is sex-mismatched, FISH chimerism studies should be considered instead.

See also:

T-cell separation


Contact Information

To contact the Molecular Haematology team please call:

Auckland City Hospital   (09) 307 4949
Lablink   ext 22000
Prof. Peter Browett (Haematologist)     ext 9090-86281
Nikhil Ghallayan (Section Leader)   ext 22006
Molecular Haematology Lab   ext 22005

For more inforamtion about the Molecular Haematology service at LabPLUS:

Molecular Haematology information page



FISH
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Cytogenetics


SEX MISMATCHED TRANSPLANT SPECIMENS

This test is performed using Fluorescent in situ hybridization (FISH).  For specimen collection details please refer to:

Chromosome Analysis -Bone Marrow Test Page




Information on Chimerism Studies:


Last updated at 11:48:27 18/03/2021