SEX MATCHED TRANSPLANT SPECIMENS
Note: All samples should be forwarded to LabPlus at room temperature within 24hours.
4 mL CPD Blood (Preferred) 4 mL CPD Bone Marrow (Preferred) 4 mL EDTA Blood 4 mL EDTA Bone Marrow 0.5 mL Paediatric Micro-EDTA Blood 0.5 mL Paediatric Micro-EDTA Bone Marrow Turnaround Time: Within 2 weeks, 4 days Diagnostic Use and Interpretation
This test uses DNA profiling to determine the contribution of donor or recipient DNA within a blood or bone marrow sample (although conceivably any sample from which DNA may be extracted could be used in this test, including blood enriched for Lymphoid (CD3+) cells (see link below)) after a patient has undergone a bone marrow transplant. The DNA profiling is performed using a panel of usually 16 different loci. A percentage contribution is calculated for each loci and the average is taken to give the percentage donor and/or recipient content.
DNA Chimerism studies will be required following a sex-matched bone marrow transplant. Please submit a second CPD tube with the Donor Storage specimen, if this service is clinically indicated.
If a bone marrow transplant is sex-mismatched, FISH chimerism studies should be considered instead.
To contact the Molecular Haematology team please call:
Auckland City Hospital (09) 307 4949 Lablink ext 22000 Prof. Peter Browett (Haematologist) ext 9090-86281 Nikhil Ghallayan (Section Leader) ext 22006 Molecular Haematology Lab ext 22005
For more inforamtion about the Molecular Haematology service at LabPLUS:
Molecular Haematology information page
SEX MISMATCHED TRANSPLANT SPECIMENS
This test is performed using Fluorescent in situ hybridization (FISH). For specimen collection details please refer to:
Chromosome Analysis -Bone Marrow Test Page