Haemolysis (rupture of red blood cells) may occur

(a) in the body (in vivo haemolysis) or

(b) during or after taking the blood sample (in vitro haemolysis).

To minimise in vitro haemolysis , take the blood with a wide-bore needle and a syringe. Draw the blood slowly using minimum suction. Avoid using a vacutainer.

Tests for in-vivo haemolysis :

• Full Blood Count including compulsory blood film
• Reticulocyte count
• Plasma haptoglobin
• LDH
• Bilirubin
• Direct Antiglobulin Test

Please note that certain tests are performed in different departments.

For Plama haptoglobin, LDH and bilirubin:- tests are performed in Biochemistry.

For FBC, Blood Film and Reticulocyte count, test is performed in Haematology.

Direct Antiglobulin Test is performed in Blood Bank.

Refer to the links below for collection and test details.

Bilirubin

Direct Antiglobulin Test

Full Blood count

Haptoglobin

LDH

reticulocyte count