Specimen Collection

Sample collection times:

Random Concentration for Area Under the Curve (AUC) monitoring	Preferred	2 -8 hours after the end of infusion Record time the blood was taken
Trough	For neonates or if AUC monitoring not available	30 minutes before next dose
Peak	-	Following I.V. bolus : 15 minutes post dose. Following I.V. infusion : 1 hour after commencement of infusion. Following I.M. injection : 1 hour post dose.

Peak levels not required for once daily dosing under normal circumstances.

To prevent possible delays requesting clinician please phone 22118 if sample sent to the laboratory out of hours.

Sample stability:

• 1 week at 2-8 ^o C
• 4 weeks at -20 ^o C

4.5 mL PST Blood (Preferred)

0.5 mL Paediatric Micro-PST Blood (Preferred)

5 mL Heparin Blood

4 mL Plain Blood

3.5 mL SST Blood

0.5 mL Paediatric Microsample Blood

0.6 mL Paediatric Micro-heparin Blood

0.5 mL Paediatric Micro-SST
Reference Intervals

Recommended monitoring:

AUC monitoring (except NICU): contact pharmacy to perform AUC calculations and advise on next dose.

Recommended levels for single daily dosing:

_	Adults	Children	Neonates
Trough	< 1 mg/L	< 0.5 mg/L	< 1 mg/L
Peak	not applicable	not applicable	not applicable

Recommended levels for multiple daily dosing (8 hourly dosing):

Trough	< 2 mg/L
Peak	5 - 10 mg/L

Uncertainty of Measurement: 10% below the level of 1.0 mg/L

8% at the levels of 5mg/L and higher

Turnaround Time: Within 3 hours
Assay Method

Principle: Colourimetric

Reagents: Roche Cedia Gentamicin II

Analyser: Cobas c502

Contact Information

For any analytical inquires, contact the Chemistry Department via LabLink or a Chemical Pathologist:

Dr Samarina Musaad (Clinical Lead) : SamarinaM@adhb.govt.nz ext. 22402

Dr Cam Kyle: CampbellK@adhb.govt.nz ext 22052

Dr Weldon Chiu: WeldonC@adhb.govt.nz ext. 23427

Dr Campbell Heron: CHeron@adhb.govt.nz ext. 23427

Dr Sakunthala Jayasinghe: Sakunthala@adhb.govt.nz ext. 23427

For any clinical and dosage inquires, contact either the antimicrobial stewardship pharmacist, the Microbiology Department via LabLink or a Clinical Microbiologist:

Dr Sally Roberts , Microbiologist: ext 22705 Cellphone 021 674 140
Dr Sharmini Muttaiyah , Microbiologist: ext 22700 Cellphone 021 615 892
Dr Mary de Almeida , Microbiologist: ext 22700 Cellphone 021 170 9117

Dr Matthew Blakiston , Microbiologist: contact via Lablink

Last updated at 08:43:34 01/04/2025