Micro collect tubes accepted for Auckland Hospital patients ONLY . All micro collects MUST be EDTA .
Must be dedicated sample ie. no other testing required.
Do not freeze unseparated PPT tubes . If the specimen requires freezing, the supernatant must be removed from the gel first.
- Require a dedicated PPT tube specimen (no other tests requested).
- Transport at 2 - 8 degrees C if transport time from collection is less than 5 days.
- Specimens need to be separated and frozen after 5 days. Unseparated specimens are unsuitable for analysis after this time.
- Absolute minimum volume required is 3.5 mL
- Require a dedicated SST tube specimen (no other tests requested).
- Centrifuge SST tube within 24 hours of collection.
- Transport at 2 - 8 degrees C if transport time from collection is less than 72 hours. Transport frozen if more than 72 hours.
- Hepatitis B virus mutation detection can be performed on this specimen.
Limit of detection for 650 ul sample volume: 10 - 1.00 +E09 (log 1.0 - 9.0) HBV DNA IU/mL
Limit of detection for 350 ul sample volume: 25 - 1.00 +E09 (log 1.4 - 9.0) HBV DNA IU/mL
One HBV IU is equivalent to 5.82 HBV DNA copies.
Uncertainty of Measurement:
+/- 0.1 log copies/ml
.
Testing is performed using the Roche Cobas 6800 HBV test.
Require a dedicated SST tube specimen (no other tests requested).
On the arrival of SST tube in the laboratory - centrifuge SST tube within 24 hours of collection.
- If testing is performed within 5 days, the sample should be stored at 4 0 C
- If the volume of plasma is less than required amount, transfer collected plasma to a conical microfuge tube prior to 600 x g centrifugation for 5 minutes. Recover remaining plasma without disturbing the pellet.