The samples must be serum , spun and frozen within an hour of collection. The samples must be transported in dry ice and frozen.
The volume required is two separate tubes of 500 micro litres each (2 x 500uL)
Radial Immunodiffusion
Complement pathway assays will indicate gross deficiencies in a pathway. They will not identify the deficient component(s) or detect relatively minor deficiencies. Used in conjunction with the other complement tests they will assist in indicating the current status of the patient's complement system.
Serum complement activity increases or decreases with various diseases such as autoimmune diseases, liver diseases, and cancers. It is recommended to measure the serum complement activity for patients who carry or are suspected to carry the diseases shown in the following table.
|
Total complement activity |
|
|
Disease where Activity is Elevated |
Disease where Activity is Reduced |
|
Chronic rheumatoid arthritis Malignant tumour Acute and chronic infection Polyarteritis Behcet's disease Systemic sclerosis Dermatomyositis Sarcoidosis Wegener's granulomatosis Multiple myeloma Reiter's syndrome |
Systemic lupus erythematosus, Malignant rheumatoid arthritis Juvenile rheumatoid arthritis Autoimmune haemolytic anaemia Serum sickness Hereditary angioneurotic oedema Acquired C1inh deficiency Disseminated intravascular coagulation Cryoglobulinaemia Felty's syndrome Malaria Membranoproliferative glomerulonephritis Acute glomerulonephritis Endotoxin shock Parietal lypodystrophy Haemodialysis Factor I deficiency Cirrhosis Fulminant hepatitis Hereditary complement deficiencies |
References
1. Mancini, G, Vaerman, J P et al. (1964). Protides of the biological fluids.(XI Colloquium). Peters H. (ed.), Amsterdam, Elsevier Publishing Co.,370-373.
2. Mancini, G, Carbonara, A O and Heremans, JF (1965). Immunochemical quantitation of antigens by single radial immunodiffusion, Immunochem 2, 235-254.
3. Fahey, J L & McKelvey, E M (1965). Quantitative determination of serum immunoglobulins in antibody-agar plates. J. Immunol., 94, 84-90.
4. Clinical medicine 3 rd edition, Parveen Kumar and Michael Clark. Publisher Baillere Tindall 1994.
For further information contact the laboratory (09) 307 4949 ext 22000 or:
Associate Professor Rohan Ameratunga , Immunopathologist: Locator 93-5724
Or the LabPLUS Immunology Team
The samples must be serum , spun and frozen within an hour of collection. The samples must be transported in dry ice and frozen.
The volume required is two separate tubes of 500 micro litres each (2 x 500uL)