Minimum of 2ml in preservative free heparin. Collection tubes are available on request from the Cytogenetics Laboratory. Mix well to avoid clotting.
For testing to be initiated, >10% plasma cells must be present in the sample.
Urgent cases: 5 days
Plasma cells are isolated using the EasySepTM Human CD138 positive selection kit.
The plasma cells are targeted with tetrameric antibody complexes recognising CD138 and magnetic particles. The labelled cells are then separated using a magnet. CD138 antigen is expressed on normal and malignant plasma cells.
The number and quality of cells examined are based on the NPAAC guidelines, unless otherwise stated.
Limitations: FISH findings should never be used in isolation to establish a diagnosis. Any signal detected below the threshold is not reported. FISH studies target specific chromosome sequences, therefore the probes used in this study will only detect abnormalities at the loci specified.
This test is designed for diagnostic bone marrow specimens from patients with multiple myeloma or other plasma cell proliferative disorders. It is not an appropriate test to track the patient's response to treatment.
Myeloma FISH consists of a panel of FISH probes which includes:
- 14q32 IGH Break-apart probe
Reflex testing will be performed if IGH is abnormal to try and identify the gene fusion partner using the the below probes:
- t(11;14)(q13;q32) CCND1-MYEOV/IGH df probe,
- t(4;14)(p16.3;q32) FGFR3-WHSC1 (MMSET)/IGH df probe, t(14;16)(q32;q23) IGH/MAF df probe
- t(14;20)(q32;q12) IGH/MAFB df probe.
- 11q22.3 ATM/ 17p13.1 TP53 gene probe (Vysis)
- 13q14 D13S319/12p11-q11 CEP12 gene probe (Vysis)
- 1q21 CKS1B/1p32.2 CDK2NA gene probe (Cytocell)
To contact the Cytogenetics team:
| Auckland City Hospital | (09) 307 4949 |
| Lablink | ext 22000 |
| Diagnostic Genetics General Enquiries | ext 22008 |
| Cytogenetics Office | ext 22012 |
All samples must be collected using sterile technique
Same day delivery (>8 degrees Celsius to <24 degrees Celsius).