Requests for this test must be arranged by contacting a LabPLUS Haematologist. Contact details below.
Requests received with no prior discussion will not be processed. Please provide requestor contact phone details on request form.
The sample must be received in the laboratory Monday - Friday 0800:1200 hrs.
Separate sample for Soluble CD25; cannot be shared with Chemistry.
Internal : Immediately spin and separate plasma to 2 x 1ml aliquots. Hand deliver spun and separated Heparin plasma to Coagulation Lab. Freeze at -20c.
External Laboratories must FREEZE the plasma within 24-hours of collection and send 2 x 1ml Heparin Plasma aliquots to LabPlus frozen.
Uncertainty of Measurement: 20%
| Age Range | Either Sex |
|---|---|
| < 2 y | < 7,887 |
| 2 y - 7 y | < 4,321 |
| >= 7 y | < 2,678 |
Requests for Soluble CD25 must be arranged through LabPlus Haematologist who will advise when results will be available. The assay takes 4 hours to process. Samples must be received in the laboratory by mid-day 12:00.
Samples were tested using Quantikine R Human CD25/IL-2Ra Immunoassay which is a 3.5 hour solid phase ELISA designed to measure soluble human IL-2Ra in cell cultures ,serum and Heparin plasma. It contains NS0 expressed recombinant human IL-2Ra and antibodies raised against the recombinant factor. Results obtained using natural Human IL-2Ra showed linear curves that were parallel to the standard curves obtained using the Quantikine R kit standard indicating this kit can be used to determine relative mass values for natural human IL-2Ra. This assay employs the quantitative sandwich enzyme immunoassay technique .A monoclonal antibody specific for human IL-2Ra has been pre coated onto a microplate. Standards, samples, and conjugate are pipetted into the wells and any IL-2Ra present is sandwiched by the immobilized antibody and the enzyme linked polyclonal antibody specific for human IL-2Ra.
Elevated serum level of IL2 Receptor alpha ( IL-2Ra ) is important in the diagnosis of HLH (Haemophagocytic Lymphohistiocytosis), which can be used to monitor treatment and to evaluate disease activity.
For further information contact the Haematology laboratory (Ext 22067) or:
| Dr Nicola Eaddy | Ext 22005 | |
| Dr Peter Bradbeer | Ext 22062 | |
| Dr Anna Ruskova | Ext 22137 | |
| Dr Nikhil Rabade | Ext 22071 |
External Laboratories must FREEZE the plasma within 24 hours of collection and send 2 x 1ml Heparin plasma aliquots to Lab Plus Frozen.