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Bone Marrow Transplant Donor Storage
Short Description : Indefinite storage for bone marrow transplant (BMT) donor specimens

Specimen Reception is occasionally asked to store bone marrow transplant (BMT) donor specimen long term. These specimens may be required at a later date for follow-up testing, in case the recipient develops complications. Specimens often come from international sources so a variety of tube types may be received.

The procedure outlined below is to be followed to ensure that Specimen Reception has comprehensive record of this storage. This record can then be used to review the storage requirements and contact the appropriate clinician.

Key contacts:
Lead BMT Physician
Dr Nigel Patton
0212409390

BMT Nurse Specialist
Rosie Howard
021893970

BMT donor storage samples should always come with the standard storage form as shown below. Form should contain both Recipient and Donor details.


Registration and Specimen Handling Guide for Specimen Services staff:

1. Specimen Handling:

1.1. Register the samples using the Recipient details (from the CMS label).

1.2.Enter the Donor details in the Reference field.

1.3. Number the specimen following standard procedures. Use donor details for specimen labelling criteria.

1.4.NB: ONE POINT OF ID IS ACCEPTABLE FOR BMT STORAGE SPECIMENS.

1.5. Standard tubes received for storage are: 1x 10mL Plain (red top); 1x 10mL EDTA (purple top). An extra CPD tube may be received in addition to the Plain and EDTA tubes. [Note: cap colours might be different from international destinations].

1.6. If an extra CPD tube is received with the Bone Marrow transplant - Donor Storage form, with supporting clinical information written on the form (e.g. Please also send sample to Molecular Haematology ), Specimens staff is to process ALL tubes received for storage (except CPD tube).

1.7. Register the Plain tube and/ or EDTA tube for BMTX; and register the CPD tube for LMH .

1.8.Only centrifuge the Plain tube and / or EDTA tube, then aliquot serum into labelled pilot tube(s) for storage. DO NOT SPIN CPD TUBE.

1.9. Store the aliquot(s) in the ORGDO box in the Clinical Trial -80 o C freezer. Please use "Current box".

[NOTE: PLEASE ALIQUOT MAXIMUM VOLUME OF SERUM OR PLASMA FOR STORAGE. INITIAL AND WRITE DOWN THE TUBE TYPE ON ALIQUOT(S)].

1.10. File the primary Plain tube and/ or EDTA tubes in the Blood Science sample storage rack system. These will be discarded after 7 days as per standard procedure.

1.11. Place the CPD tube with a copy of the request form in the Special Haematology Specimen Box. Molecular Haematology will process the CPD sample for DNA extraction.

1.12. If CPD tube is received but no supporting clinical information is written on the request form; do NOT register the CPD sample, make a photocopy of the request form, and give both the form and CPD tube to the Specimens Trouble-shooter. Specimens Trouble-shooter will refer request form and CPD tube to Mol. Haem staff for clarification.

1.13. For after-hours request, do NOT register CPD tube, place CPD tube and copy of request form into the room temperature trouble-shooter box for trouble-shooter to deal with the following day.



Sample type(s) Registration code used Sample handling Storage
Plain (Red) / EDTA (Purple) BMTX Centrifuge and aliquot serum into labelled pilot tube(s) for storage. Aliquot tube(s) - In -80 o C Freezer (Indefinite).
Primary tube(s) - In Blood Science sample storage rack system (Discard after 7 days).
CPD tube(s) LMH DO NOT SPIN CPD tube(s).
Place the CPD tube(s) with a copy of request form in the Special Haematology Specimen Box. 		Sample(s) are processed by Molecular Haematology section.



Last updated at 11:15:26 20/12/2019