Requests for this test must be arranged by contacting a LabPLUS Haematologist.
If requests are received with no prior discussion they will not be processed.
Please provide requestor contact phone details on the request form.
NOTES:
1. EDTA destroys ADAMTS-13 activity so
it is
important to collect/fill citrate specimen(s) before EDTA specimens to avoid EDTA contamination.
2. Collection tubes must be adequately filled.
Reference range >40%
| ADAMTS-13 ACTIVITY LEVEL | UOM |
| Greater than 20% | 18% |
Requests for ADAMTS-13 activity must be arranged through a
LabPlus
Haematologist who will also advise when results will be available. Please note the assay takes 5 hours to complete.
As from October 2017 ADAMTS-13 Activity is determined by an ELISA chromogenic test at Labplus.
The ADAMTS-13 activity is reported together with an ADAMTS-13 inhibitor screen for timely distinction between congenital and acquired deficiency.
ADAMTS13 is an enzyme important to regulation of von Willebrand Factor-endothelial function.
Deficiency of ADAMTS-13 can be congenital or acquired through an inhibitor. Deficiency leads to devastating thrombotic episodes presenting clinically as thrombotic thrombocytopenic purpura, pregnancy complications with pregnancy loss and maternal morbidity/mortality, paediatric thrombotic stroke, myocardial infarction in young patients, and possibly cerebrovascular events in Type 1 diabetes.
Immediate treatment is through plasma therapy usually repeated plasmapheresis in a very sick patient which is risky, and also costly. Subsequent treatment in acquired cases involves immunosuppression and in congenital cases regular plasma infusion.
The test will contribute to immediate diagnosis of the condition to start appropriate treatment to improve patient outcomes.
For further information contact the Haematology laboratory (Ext 22067) or:
| Dr Nicola Eaddy | Ext 22005 | |
| Dr Peter Bradbeer | Ext 22062 | |
| Dr Anna Ruskova | Ext 22137 | |
| Dr Nikhil Rabade | Ext 22071 |
Double spin plasma before freezing in 1 ml aliquots.
To ensure the plasma is platelet poor before freezing double spin plasma as follows: transfer the plasma after the initial centrifugation to a suitable plastic tube using a plastic transfer pipette then recentrifuge. When aliquoting from the second spin tube into the final tube take care to avoid the bottom 0.5 cm where residual platelets are deposited. Identify double spun aliquots by noting this on the label.
Haemolysed specimens may be unsuitable for testing.