Specimen Collection

Samples need to be received in the laboratory within 1 hour of collection or spun and frozen within that time.

3.5 mL SST Serum (Preferred)

4 mL Plain Serum
Reference Intervals
Units: mg/L

Age Range	Either Sex
All	70 - 700

Turnaround Time: Within 5 weeks

Due to the rarity of requests for this assay, reagents are not kept in stock and will be ordered when a request is received.

Assay Method

Radial Immunodiffusion using a Binding Site kit.

Diagnostic Use and Interpretation

Factor H is a regulatory protein in the complement pathway. It may be decreased or absent as a result of mutations in the protein. It may also be decreased as a result of increased consumption or antibodies directed against factor H.

References:

1. Mancini, G, Vaerman, J P et al. (1964). Protides of the biological fluids (XI Colloquium). Peters H. (ed.), Amsterdam, Elsevier Publishing Co.,370-373.
2. Mancini, G, Carbonara, A O and Heremans, JF (1965). Immunochemical quantitation of antigens by single radial immunodiffusion, Immunochem 2, 235-254.
3. Fahey, J L & McKelvey, E M (1965). Quantitative determination of serum immunoglobulins in antibody-agar plates. J. Immunol., 94, 84-90.
4. Norman, D.G., Barlow, P.N., Baron, M., Day, A.J., Sim, R.B., Campbell, I.D. Three-dimensional structure of a complement control protein module in solution. J Mol Biol 219(4):717-725.(1991)
5. Uhrinova, S., Lin, F., Ball, G., Bromek, K., Uhrin, D., Medof, M.E., Barlow, P.N. Solution structure of a functionally active fragment of decay-accelerating factor. Proc Natl Acad Sci USA 100(8):4718-4723.(2003)
6. Szakonyi, G., Guthridge, J.M., Li, D., Young, K., Holers, V.M., Chen, X.S. Structure of complement receptor 2 in complex with its C3d ligand. Science 292(5522):1725-1728.(2001)
7. Buddles, M.R., Donne, R.L., Richards, A., Goodship, J. & Goodship, T.H. Complement factor H gene mutation associated with autosomal recessive atypical hemolytic uremic syndrome. Am J Hum Genet 66, 1721-1722 (2000).
8. Hageman, G.S. et al. A common haplotype in the complement regulatory gene factor H (HF1/CFH) predisposes individuals to age-related macular degeneration. Proc Natl Acad Sci U S A 102, 7227-7232 (2005).
   

Contact Information

For further information contact the laboratory (09) 307 4949 ext 22000 or:

Associate Professor Rohan Ameratunga , Immunopathologist: Locator 93-5724

Or the LabPLUS Immunology Team

Specimen Transport Instructions for Referring Laboratories

Spin and separate samples within 1 hour of collection.

Send frozen.

Last updated at 13:15:56 07/01/2025