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Test performed by: LabPLUS Automation

Specimen Collection

In men it is preferable to collect a morning (before 10 a.m.) sample, because of diurnal variation in serum testosterone.


4.5 mL PST Blood (Preferred)


0.5 mL Paediatric Micro-PST Blood (Preferred)


5 mL Heparin Blood


4 mL Plain Blood


3.5 mL SST Blood
Reference Intervals

Units: nmol/L



testosterone (nmol/L)

0 - 5 months

0 - 17

6 months - 9 years (prepubertal)

0 - 1.0

10 - 14 years (depends on stage of puberty)

0 - 28

15 - 19 years

7.6 - 28

20 - 50 years

8.7 - 29

>50 years

6.7 - 26



testosterone (nmol/L)

0 - 9 years

0 - 0.5

10 - 15 years

0 - 1.4


0 - 1.8

Uncertainty of Measurement: 7%

  • Testosterone levels in males are highest in the morning, and decrease thereafter. Reference intervals apply to pre-9 a.m. samples.

  • Turnaround Time: Within 1 day
    Assay Method

    Principle : Competitive immunoassay with chemiluminescence detection

    Reagents : Roche testosterone II kit

    Analyser : Cobas e602

    Diagnostic Use and Interpretation

    The total testosterone level provides an adequate assessment of testosterone status in most cases. In borderline cases free testosterone provides a slightly better estimate of androgen activity than total testosterone, because changes in sex hormone binding globulin (SHBG) are incorporated into the calculation of free testosterone. (See testosterone - free )

    Investigations for hirsutism in women (ref. 1)

    A testosterone level may be performed in the initial investigation of hirsutism, acne or menstrual irregularity.

    Mild hirsutism of slow onset without other signs of androgen excess (acne, clitoromegaly or muscularity), menstrual irregularity or infertility does not require measurement of testosterone or any other androgen.

    In women with moderate or severe hirsutism or hirsutism of any degree when it is sudden in onset, rapidly progressive, or associated with other abnormalities such as menstrual dysfunction, obesity, or clitoromegaly, a testosterone level is recommended.

    If the testosterone level is raised, the commonest cause is PCOS. To exclude other conditions the following investigations are suggested (1):

    Mildly raised levels are consistent with PCOS or idiopathic hyperandrogenism.

    Once a diagnosis has been made, repeated measurements of testosterone are not usually necessary or helpful.


    Primary hypogonadism is present if the testosterone is low, and LH and FSH are high.

    Secondary hypogonadism is present if the testosterone is low and LH and FSH are normal or low.

    What is a normal level of serum testosterone in males?

    The reference intervals shown above represent the central 95% of a normal population.

    Large cross-sectional studies of serum testosterone in healthy adult males have shown a progressive decrease of total and free testosterone with age from 25 years onwards.

    The relationship of testosterone level to sexual function is controversial. Symptoms of sexual dysfunction were associated with testosterone levels less than 8 nmol/L in one study (5) while another found levels less than 11 nmol/L were significantly associated with symptoms of sexual dysfunction (4).

    In symptomatic patients with pituitary or hypothalamic disease, total testosterone values are typically < 8 nmol/l, and associated with other hormone deficiencies (such as a low or falling T4) or a raised serum prolactin.

    Because of the diurnal variation (testosterone highest in the early morning), low results should be confirmed with an early morning sample.

    Monitoring testosterone replacement : It is not usually necessary to monitor testosterone levels in patients recieving injections of depot testosterone (e.g. Sustanon). This may be helpful if the clinical response is poor, to confirm that an adequate testosterone level has been achieved. After the injection there is a high level for several days, followed by a decline over 2 - 4 weeks, or longer depending on the preparation injected. For monitoring, a trough testosterone level should be obtained (i.e. before the next injection). The target trough level is 8 - 12 nmol/L. The interval between injections can be adjusted to achieve the target level. Once the correct interval has been determined, further testosterone tests should not be necessary.

    References :

    1. Martin KA, Chang RJ, Ehrmann DA et al. Evaluation and treatment of hirsutism in premenopausal women: an endocrine society clinical practice guideline. J Clin Endocrinol Metab. 2008;93:1105-1120

    2. Soldin et al. Paediatric Reference Ranges, 3rd ed (1999)

    3. Endocrine Sciences, Adrenal steroid responses to ACTH (in house publication, 1991)

    4. Wu FC, Tajar A, Beynon JM et al. Identification of late-onset hypogonadism in middle-aged and elderly men. N Engl J Med 2010;363:123-135

    5. O'Connor, D. B., D. M. Lee, et al. (2011). "The relationships between sex hormones and sexual function in middle-aged and older European men." J Clin Endocrinol Metab 96(10): E1577-1587.

    Contact Information

    Emails to chemicalpathologist@adhb.govt.nz will receive priority attention from the on-call chemical pathologist.

    If the query concerns a specific patient please include the NHI number in your email.

    If email is not a suitable option, please contact the on-call chemical pathologist via Lablink (Auckland City Hospital ext. 22000 or 09-3078995).

    Individual chemical pathologists may be contacted but will not be available at all times.

    Dr Cam Kyle (Clinical Head): CampbellK@adhb.govt.nz ext 22052 or mobile 027 276 0038

    Dr Weldon Chiu: WeldonC@adhb.govt.nz ext. 23427 or mobile 027 290 1073

    Dr Leah Ha: LHa@adhb.govt.nz ext. 23427 or mobile 021 308 806

    Dr Samarina Musaad: SamarinaM@adhb.govt.nz ext. 22402 or mobile 021 404 769

    Last updated at 13:30:01 19/05/2021