Specimen Collection

For samples collected within the Auckland region:

2 mL Adult Heparin Blood (Preferred)

Note: PST (light green cap) tubes are not suitable.

0.5 mL Paediatric Heparin Whole Blood (Preferred)

2 mL Adult EDTA Blood

0.5 mL Paediatric EDTA Whole Blood

• Heparin blood samples are preferred as they are stable up to 48-hours
• EDTA blood samples (2mL adult or 0.5mL paediatric) are acceptable only if received by the laboratory within 20-hours after collection.
• ACD tubes are adequate but not preferred. Ensure ACD tubes are completely filled with blood.

Samples outside Auckland region:

• Sample collection as above.
• Transport at room temperature (do not refrigerate).
• Sample age limit for testing: 48-hours (Heparin), 24-hours (EDTA).

Reference Intervals

REFERENCE INTERVAL: Lymphocyte subpopulation: cells/uL

Adult	cells/uL
CD3	780 - 2600
CD4	500 - 1650
CD8	210 - 1200
CD19	80 - 600
CD56	40 - 500

Paediatric: (cells/ul)	Neonatal	1wk - 2mths	3 - 9 mths	10 - 24 mths	2 - 5 yrs
CD3	600 - 5000	2300 - 7000	2300 - 6700	1500 - 7500	900 - 4500
CD4	400 - 3500	1700 - 5300	1400 - 5000	900 - 5000	500 - 2400
CD8	200 - 1900	400 - 1700	500 - 1900	400 - 2200	300 - 1600
CD19	400 - 1100	600 - 1900	600 - 1900	600 - 2900	200 - 2100
CD56	100 - 1900	200 - 1400	100 - 1100	200 - 1300	100 - 1000

Uncertainty Of Measurement (UOM):

	UOM for absolute results
CD3	6 %
CD4	9 %
CD8	11 %
CD19	12 %
CD56	14 %

Turnaround Time: Within 2 days
Contact Information

For further information contact:

• Cell Markers laboratory ext. 22061 or 22075.
• Via Lablink ext. 22000 or (09) 307-8995 or 0800 522 758.
• Out-of-hours on-call 021 753 059.
• Immunology@adhb.govt.nz
• Associate Professor Rohan Ameratunga, Immunopathologist; rohana@adhb.govt.nz

Last updated at 13:45:02 26/09/2024