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AML1-ETO Transcript Detection by PCR - t(8;21)
Short Description : Detection of t(8;21) AML1-ETO transcripts by PCR
Also known as : [8;21],[RT-PCR]
DNA/RNA
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Molecular Haematology
Specimen Collection
NOTE:
Do not collect samples on a Friday, the weekend or the day falling immediately prior to a long weekend. These samples may be rejected and a re-collect required.
RNA is highly unstable and specimens degrade rapidly. For optimum results samples should be sent in CPD tubes at room temperature and be received by this lab within 24 hours of collection. Although LabPlus operates 24 hours a day, 7 days a week, the Molecular Haematology section only operates 9-5, Mon-Fri (excluding public holidays). Samples arriving on a Friday afternoon or a Saturday or Sunday may not get processed until the following Monday. Such delays can significantly compromise the quality of the sample.
9 mL CPD Blood (Preferred)
2 mL CPD Bone Marrow (Preferred)
9 mL EDTA Blood
2 mL EDTA Bone Marrow
Turnaround Time: Within 2 weeks, 4 days
Diagnostic Use and Interpretation
This PCR tests for the presence of the fusion transcript resulting from the t(8;21)(q22;q22) translocation. Results can be used to aid in the diagnosis and prognosis of AML patients.
Contact Information
To contact the Molecular Haematology team please call:
For more information about the Molecular Haematology service at LabPLUS:
Molecular Haematology information page
FISH
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Cytogenetics
A FISH (fluorescent in situ hydridisation) based assay is also available for this test.
Refer to Molecular Cytogenetics Probe List
Information on AML1-ETO Translocation:
This PCR tests for the presence of the fusion transcript resulting from the t(8;21)(q22;q22) translocation. The t(8;21) forms a fusion gene made up of parts of the AML1 gene (acute myeloid leukaemia 1) located on 21q22 fused to the ETO gene located on 8q22. Transcripts generate products of a constant size corresponding to an in frame fusion of AML1 exon 5 to ETO exon 2. This transcript is found in all positive cases of t(8;21) but may also be present in complex translocations and some t(8;21)-negative AML cases.
References:
Delabesse E. et al Leukemia 1999; 13: 1908-1910
Last updated at 15:36:59 07/03/2024