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SCID Flow Panel
Also known as : [SCID Flow Panel]


Blood
Test performed by: LabPLUS Special Haematology


Specimen Collection

Room temperature. 

DO NOT SPIN. 


Micro-heparin0.5 mL Paediatric Micro-heparin Whole Blood (Always Required)
Micro-EDTA0.5 mL Paediatric Micro-EDTA Blood (Always Required)
Reference Intervals

 

CD4+ Naive T-cells

  (CD45RA+CD62L+)%

CD4+ Naive T-cells absolutes x10 6 /L

CD8+ Na?ve T-cells

  (CD45RA+CD62L+)%

CD8+ Naive T-cells absolutes x10 6 /L

0 - 3 months

61 - 94

1200 - 3600

56 - 88

380 - 1300

3 - 6 months

64 - 92

1300 - 3600

53 - 88

450 - 1200

6 - 12 months

58 - 91

1100 - 3600

47 -87

330 - 1200

1 - 2 years

62 - 90

950 - 2800

46 - 85

400 - 1400

2 - 6 years

50 - 85

420 - 1500

42 - 81

260 - 850

6 - 12 years

42 - 74

310 -1000

39 - 73

200 - 650

12 - 18 years

31 - 65

210 - 750

42 - 73

170 - 560

 

CD4+ Memory T-cells %

CD4+ Memory T-cells absolutes x10 6 /L

CD8+ Memory T-cells %

CD8+ Memory T-cells absolutes x10 6 /L

0 - 3 months

2 - 22

60 - 900

1 - 9

30 - 330

3 - 6 months

3 - 16

120 - 630

1 - 7

30 - 290

6 - 12 months

5 - 18

160 - 800

1 - 8

40 - 330

1 - 2 years

7 - 20

210 - 850

2 - 12

60 - 570

2 - 6 years

9 - 26

220 - 660

4 - 16

90 - 440

6 - 12 years

13 - 30

230 - 630

4 - 21

70 - 390

12 - 18 years

18 - 38

240 - 700

4 - 23

60 - 310

* Ref ranges from Shearer et. al. Lymphocyte subsets in healthy childrenfrom birth through 18 years of age: The Pediatric AIDS Clinical Trials GroupP1009 study. J Allergy Clin Immunol. 2003; 112 number 5: 975-979.



Turnaround Time: Within 1 day
Assay Method

Flow Cytometry.


Diagnostic Use and Interpretation

For the investigation of SCID in newborn babies.

Lymphocyte Subsets , Naive T-cells

Lymphoctye Subsets

Memory and Naive T- Cell


Contact Information

Associate Professor Rohan Ameratunga, Immunologist ( rohan.ameratunga@adhb.govt.nz ) or the Immunlogy Team (Immunology@adhb.govt.nz)


Specimen Transport Instructions for Referring Laboratories

Sample collection

Sample dispatch

  Notify Cell Marker section Haematology LabPlus (09 307 8995 ext 22061) once sample has been dispatched.

If concerns, contact the on call paediatric immunologist at Starship Children's Hospital, Auckland City Hospital, through the operator on 09 367 0000 * , or the Newborn Screening Laboratory LabPlus on 021745847 during working hours (Monday to Fridays, 0800 - 1700). 



Last updated at 08:43:31 13/09/2018