Note: All samples should be forwarded to LabPlus at room temperature within 24hours.
4 mL CPD Blood (Preferred) 4 mL EDTA Blood Turnaround Time: Within 2 weeks, 4 days Diagnostic Use and Interpretation
This test detects a common variant in the Prothrombin gene. Results can be used to establish a heritable cause for an increased risk of thrombosis.
Factor V Leiden mutation analysis
Diagnostic Use and Interpretation Contact Information
To contact the Molecular Haematology team please call:
Auckland City Hospital (09) 307 4949 Lablink ext 22000 Prof. Peter Browett (Haematologist) ext 9090-86281 Nikhil Ghallayan (Section Leader) ext 22006 Molecular Haematology Lab ext 22005
For more inforamtion about the Molecular Haematology service at LabPLUS:
Molecular Haematology information page
A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Up to 18% of patients with a family history of venous thrombosis have been reported to be carriers of this variant as compared to approximately 1% in the general population. Heterozygous patients have a 3 - 4 fold increased risk of thrombosis. The prothrombin 20210A allele is detected by PCR of a 169bp product of the 3'-untranslated region of the gene using a downstream mutagenic primer which introduces a Hind III site in the presence of the A allele.
Poort SR et al. Blood 88: 3698-3703; 1966