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PML-RARalpha Transcript Detection by PCR
Also known as : [15;17],[RT-PCR],[t(15;17)]


DNA/RNA
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Molecular Haematology


Specimen Collection

NOTE: RNA is highly unstable and specimens degrade rapidly. For optimum results samples should be sent in CPD tubes at room temperature and be received by this lab within 24 hours of collection. Although LabPlus operates 24 hours a day, 7 days a week, the Molecular Haematology section only operates 9-5, Mon-Fri (excluding public holidays). Samples arriving on a Friday afternoon or a Saturday or Sunday may not get processed until the following Monday. Such delays can significantly compromise the quality of the sample. 


CPD9 mL CPD Blood (Preferred)
CPD2 mL CPD Bone Marrow (Preferred)
EDTA9 mL EDTA Blood
EDTA2 mL EDTA Bone Marrow
Turnaround Time: Within 2 weeks, 4 days
Diagnostic Use and Interpretation

This PCR tests for the presence of the fusion transcript resulting from the t(15;17) (q22;q21) chromosomal translocation.


Contact Information

To contact the Molecular Haematology team please call:

Auckland City Hospital   (09) 307 4949
Lablink   ext 22000
Prof. Peter Browett (Haematologist)     ext 9090-86281
Nikhil Ghallayan (Section Leader)   ext 22006
Molecular Haematology Lab   ext 22005

For more inforamtion about the Molecular Haematology service at LabPLUS:

Molecular Haematology information page



FISH
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Cytogenetics


A FISH (fluorescent in situ hydridisation) based assay is also available for this test.

Refer to Molecular Cytogenetics Probe List




Information on the PML-RARalpha Translocation:

This PCR tests for the presence of the fusion transcript resulting from the t(15;17) (q22;q21) chromosomal translocation.  Primers were designed by the European BIOMED-1 Concerted Action group.  This group was comprised of 14 laboratories from eight European countries who had the aim of setting up an international standardization of the protocols for the investigation of minimal residual disease in acute leukaemia. 

References:
Biondi A. et al Leukemia 1999; 13: 1915-1917


Last updated at 11:48:27 18/03/2021