NOTE: RNA is highly unstable and specimens degrade rapidly. For optimum results samples should be sent in CPD tubes at room temperature and be received by this lab within 24 hours of collection. Although LabPlus operates 24 hours a day, 7 days a week, the Molecular Haematology section only operates 9-5, Mon-Fri (excluding public holidays). Samples arriving on a Friday afternoon or a Saturday or Sunday may not get processed until the following Monday. Such delays can significantly compromise the quality of the sample.
9 mL CPD Blood (Preferred) 2 mL CPD Bone Marrow (Preferred) 9 mL EDTA Blood 2 mL EDTA Bone Marrow Turnaround Time: Within 2 weeks, 4 days Diagnostic Use and Interpretation
This PCR tests for the presence of the fusion transcript resulting from the t(15;17) (q22;q21) chromosomal translocation.
To contact the Molecular Haematology team please call:
Auckland City Hospital (09) 307 4949 Lablink ext 22000 Prof. Peter Browett (Haematologist) ext 9090-86281 Nikhil Ghallayan (Section Leader) ext 22006 Molecular Haematology Lab ext 22005
For more inforamtion about the Molecular Haematology service at LabPLUS:
Molecular Haematology information page
A FISH (fluorescent in situ hydridisation) based assay is also available for this test.
Refer to Molecular Cytogenetics Probe List
This PCR tests for the presence of the fusion transcript resulting from the t(15;17) (q22;q21) chromosomal translocation. Primers were designed by the European BIOMED-1 Concerted Action group. This group was comprised of 14 laboratories from eight European countries who had the aim of setting up an international standardization of the protocols for the investigation of minimal residual disease in acute leukaemia.
Biondi A. et al Leukemia 1999; 13: 1915-1917