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Memory and Naive T-Cell assay
Also known as : [Naive T cells],[T Cell Assay - Memory and Naive Cells]


Blood
Test performed by: LabPLUS Cell Markers


For all queries, including specimen requirements and results, contact the Cell Marker section of the Haematology laboratory ( Ext22061 or 22075),

or via Lablink   22000 or (09) 307-8995 or 0800 522 758 .


Specimen Collection
Heparin4 mL Adult Heparin Blood

Dark green top Heparin tubes are required ,

light green top PST Heparin tubes(containing gel) are NOT suitable.

 

or


Heparin1 mL Paediatric Heparin Blood

Dark green top Heparin tubes are required ,

light green top PST Heparin tubes(containing gel) are NOT suitable.

 

For samples sent to LabPlus from outside the Auckland region:


Turnaround Time: Within 1 week, 3 days
Diagnostic Use and Interpretation

  1. To determine the phenotypic severity of DiGeorge Syndrome.
  2. Selected bone marrow transplant recipients to determine engraftment.
  3. Novel Immune deficiency disorders.
  4. Selected rheumatoid arthritis patients.
  5. Selected HIV patients.

CD45RA and CD62L are used to identify naive T-cell subsets, CD45RO and CD62L are used to identify memory T-cell subsets. The following phenotypes have been described in literature:

CD4+ T helper naive cells:                CD4+CD45RA+CD62L+


CD4+ T helper memory cells:           CD4+CD45RO+CD62L+
                                                                CD4+CD45RO+CD62L-
                                                                CD4+CD45RA+CD62L- (very small subset)

CD8+ T cytotoxic naive cells:            CD8+CD45RA+CD62L+


CD8+ T cytotoxic memory cells:        CD8+CD45RO+CD62L+
                                                                 CD8+CD45RO+CD62L-

                                                                 CD8+CD45RA+CD62L-


Contact Information

For further information contact the Cell Markers laboratory (ext 22061) , or out of hours on-call: 021 753 059 , or :


Associate Professor Rohan Ameratunga , Immunologist:

or the Immunology Team



Last updated at 14:41:15 28/04/2013