Note: Bone marrow aspirate is the preferred specimen type for this assay. Peripheral blood should only be considered if bone marrow is unobtainable and there are known to be sufficient lymphoplasmocytic cells in circulation for analysis.
3 mL CPD Bone Marrow (Preferred) 5 mL EDTA Blood 3 mL EDTA Bone Marrow 5 mL CPD Blood Turnaround Time: Between 2 weeks and 4 weeks Diagnostic Use and Interpretation Recurrent activating mutations within MYD88 have been identified in certain sub-types of B-cell lymphomas. The c.794T>C (L265P) mutation in exon 5 of the MYD88 gene is seen in >90% of patients with Waldenstrom's macroglobulinemia (IgM secreting lymphoplasmacytic lymphoma) and non-IgM-secreting lymphoplasmacytic lymphoma (LPL) [N Eng Med 2012;367:826-833]. MYD88 mutations have also been reported in up to 10-30% of other lymphomas of B-cell origin such as; ABC-DLBCL, GCB-DLBCL, CLL and MALT lymphomas. The c.794T>C mutation can be detected by allele specific PCR with a sensitivity of 0.1% using the method of Varettoni et al. Blood 2013;121(13): 2522-2528. Contact Information
To contact the Molecular Haematology team please call:
Auckland City Hospital (09) 307 4949 Lablink ext 22000 Prof. Peter Browett (Haematologist) ext 9090-86281 Nikhil Ghallayan (Section Leader) ext 22006 Molecular Haematology Lab ext 22005
For more inforamtion about the Molecular Haematology service at LabPLUS:
Molecular Haematology information page