NOTE: RNA is highly unstable and specimens degrade rapidly. For optimum results samples should be sent in CPD tubes at room temperature and be received by this lab within 24 hours of collection. Although LabPlus operates 24 hours a day, 7 days a week, the Molecular Haematology section only operates 9-5, Mon-Fri (excluding public holidays). Samples arriving on a Friday afternoon or a Saturday or Sunday may not get processed until the following Monday. Such delays can significantly compromise the quality of the sample.
9 mL CPD Blood (Preferred) 2 mL CPD Bone Marrow (Preferred) 9 mL EDTA Blood 2 mL EDTA Bone Marrow Turnaround Time: Within 2 weeks, 4 days Diagnostic Use and Interpretation
This test detects the number of BCR-ABL transcripts in a blood or bone marrow sample and normalises this against an endogenous control gene (BCR). These results are used to monitor a patient's response to treatment. A 3 log reduction in BCR-ABL levels after 12 months of treatment is correlated with a decreased risk of disease progression over 5 years.
The sensitivty of the Q-PCR method is sufficiently high that it is possible to detect potentially clinically relevant increases in BCR-ABL transcript levels before the number of Ph+ cells is high enough to be detected by other monitoring methods (conventional cytogenetics or FISH studies).
Brian J. Druker et al., The New England Journal of Medicine, 2006, 355(23), p2408.
BCR-ABL Transcript Detection by RT-PCR
BCR-ABL Tyrosine Kinase Sequencing
To contact the Molecular Haematology team please call:
Auckland City Hospital (09) 307 4949 Lablink ext 22000 Prof. Peter Browett (Haematologist) ext 9090-86281 Nikhil Ghallayan (Section Leader) ext 22006 Molecular Haematology Lab ext 22005
For more inforamtion about the Molecular Haematology service at LabPLUS:
Molecular Haematology information page
A FISH (fluorescent in situ hydridisation) based assay is also available for this test.
Refer to Molecular Cytogenetics Probe List