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BCR-ABL Analysis by Q-PCR
Short Description : Quantitation of BCR-ABL transcript levels by Q-PCR
Also known as : [Q-PCR],[Quantitative BCR-ABL Analysis]


DNA/RNA
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Molecular Haematology


Specimen Collection

NOTE: RNA is highly unstable and specimens degrade rapidly. For optimum results samples should be sent in CPD tubes at room temperature and be received by this lab within 24 hours of collection. Although LabPlus operates 24 hours a day, 7 days a week, the Molecular Haematology section only operates 9-5, Mon-Fri (excluding public holidays). Samples arriving on a Friday afternoon or a Saturday or Sunday may not get processed until the following Monday. Such delays can significantly compromise the quality of the sample. 


CPD9 mL CPD Blood (Preferred)
CPD2 mL CPD Bone Marrow (Preferred)
EDTA9 mL EDTA Blood
EDTA2 mL EDTA Bone Marrow
Turnaround Time: Within 2 weeks, 4 days
Diagnostic Use and Interpretation

This test detects the number of BCR-ABL transcripts in a blood or bone marrow sample and normalises this against an endogenous control gene (BCR).  These results are used to monitor a patient's response to treatment. A 3 log reduction in BCR-ABL levels after 12 months of treatment is correlated with a decreased risk of disease progression over 5 years.

The sensitivty of  the Q-PCR method is sufficiently high that it is possible to detect potentially clinically relevant increases in BCR-ABL transcript levels before the number of Ph+ cells is high enough to be detected by other monitoring methods (conventional cytogenetics or FISH studies).  

References:
Brian J. Druker et al., The New England Journal of Medicine, 2006, 355(23), p2408.

See also: 

BCR-ABL Transcript Detection by RT-PCR

BCR-ABL Tyrosine Kinase Sequencing


Contact Information

To contact the Molecular Haematology team please call:

Auckland City Hospital   (09) 307 4949
Lablink   ext 22000
Prof. Peter Browett (Haematologist)     ext 9090-86281
Nikhil Ghallayan (Section Leader)   ext 22006
Molecular Haematology Lab   ext 22005

For more inforamtion about the Molecular Haematology service at LabPLUS:

Molecular Haematology information page



FISH
Test performed by: LabPLUS - Dept. Diagnostic Genetics - Cytogenetics


A FISH (fluorescent in situ hydridisation) based assay is also available for this test.

Refer to Molecular Cytogenetics Probe List




Information on Quantitative PCR for BCR-ABL transcripts:


Last updated at 11:48:27 18/03/2021