NOTE: RNA is highly unstable and specimens degrade rapidly. For optimum results samples should be sent in CPD tubes at room temperature and be received by this lab within 24 hours of collection. Although LabPlus operates 24 hours a day, 7 days a week, the Molecular Haematology section only operates 9-5, Mon-Fri (excluding public holidays). Samples arriving on a Friday afternoon or a Saturday or Sunday may not get processed until the following Monday. Such delays can significantly compromise the quality of the sample.
9 mL CPD Blood (Preferred) 2 mL CPD Bone Marrow (Preferred) 9 mL EDTA Blood 2 mL EDTA Bone Marrow Turnaround Time: Within 2 weeks, 4 days Diagnostic Use and Interpretation
This PCR tests for the presence of the fusion transcript resulting from the t(8;21)(q22;q22) translocation. Results can be used to aid in the diagnosis and prognosis of AML patients.
To contact the Molecular Haematology team please call:
Auckland City Hospital (09) 307 4949 Lablink ext 22000 Prof. Peter Browett (Haematologist) ext 9090-86281 Nikhil Ghallayan (Section Leader) ext 22006 Molecular Haematology Lab ext 22005
For more inforamtion about the Molecular Haematology service at LabPLUS:
Molecular Haematology information page
A FISH (fluorescent in situ hydridisation) based assay is also available for this test.
Refer to Molecular Cytogenetics Probe List
This PCR tests for the presence of the fusion transcript resulting from the t(8;21)(q22;q22) translocation. The t(8;21) forms a fusion gene made up of parts of the AML1 gene (acute myeloid leukaemia 1) located on 21q22 fused to the ETO gene located on 8q22. Transcripts generate products of a constant size corresponding to an in frame fusion of AML1 exon 5 to ETO exon 2. This transcript is found in all positive cases of t(8;21) but may also be present in complex translocations and some t(8;21)-negative AML cases.
Delabesse E. et al Leukemia 1999; 13: 1908-1910