Clinical details are required to establish the appropriate testing strategy for the patient, and to enable the report/s to be cross referenced to other tests that have been performed.
Specimen Requirements for Formalin Fixed Paraffin Embedded (FFPE) Tissues:
Requests for FISH testing can be made using the Diagnostic Genetics request form found on the Lab Plus website and Test Guide.
6 x slides of ~4 um thickness paraffin embedded tissue.
1 x H&E stained slide with the area of interest marked.
All specimens should be processed through a Histopathology laboratory.
All specimens sent must be accompanied by the following:
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A request form with working diagnosis and probe(s) requested.
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Two forms of matching identification on both slides and the form.
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A marked H+E slide with the area/s to be targeted
If multiple areas are targeted, please indicate whether they need to be analyzed separately. -
A copy of the histology report.
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Additional relevant information .
This includes whether the specimen has been decalcified or has undergone additional histological processing,
areas of different morphology within the tumour sample, and the presence of more than one tumour within the sample. -
HER2 IHC status for ERBB2/HER2 FISH.
In case of an equivocal HERFISH result, the report will not be released without IHC score.
In cases where inadequate details are provided, a report will be issued to indicate that inadequate details were received with request.
Failure to include relevant information may result in a reporting delay, as consultation with LabPlus pathologists may be required.
Specimen Requirements for non-formalin fixed tissue:
Please use the links below:
See also:
See Chromosome Analysis - Amniotic Fluid and Chorionic Villus specimens
See Chromosome Analysis - Blood
Tests available and TAT
At LabPlus, Fluorescence In Situ Hybridisation (FISH) studies are done on
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Formalin Fixed Paraffin Embedded Tissue (FFPET) slides
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Non formalin fixed tissue/Suspension samples (bone marrow, blood and prenatal samples)
Use the link below to view full list of currently available FISH probes and Turn Around Time (TAT) for FFPET and suspension samples.
1. FISH Tests performed on FFPET:
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* Working days. All listed TATs are a given as a guide only. NPAAC guidelines allow a TAT of 18 calendar days.
BA = Break apart probe, DF = Dual fusion probe, SF = Single fusion probe.
FISH tests on non-formalin fixed samples/suspension samples:
Use the link below to view full list of currently available FISH probes and Turn Around Time (TAT) for bone marrow, blood, and prenatal samples.
To contact the Molecular Genetics team:
Auckland City Hospital
(09) 307 4949
Lablink
ext 22000
Sajayan Joseph (Section Leader)
ext 22007
All specimens for FFPET FISH should be processed through a Histopathology laboratory.