Sample must be centrifuged within 60 minutes of collection.
Separated fluoride plasma is stable for
- 8 hours at 15-25 o C
- 14 days at 2-8 o C
DO NOT USE SERUM
Paediatric Microcollect Capillary Blood Gas
For capillary sample: Collect one heparinised capillary tube.
Mix well. Transport to laboratory without delay.
Capillary samples are very dependent on the quality of specimen collection, including the maintenance of anaerobic conditions during the procedure, to produce reliable results.
Sample must be centrifuged within 60 minutes of collection.
Units: mmol/L
Reference interval:
Venous and Arterial: <2.0 mmol/L (at rest)
Cord blood: <6.1 mmol/L
Note:
Locally agreed "action limits" have also been set for other hand-held meters for neonates (<3 mmol/L), and for fetal scalp pH during delivery (<4.2 mmol/L) (see Table below). These are alert thresholds and not reference limits.
Uncertainty of Measurement: 5%
** For Whole Blood Uncertainty of measurement refer to the Blood Gases page
Principle: Enzymatic
Reagents: Roche Lactate kit
Analyser: Cobas c702
Lactate concentrations may be increased in:
A. Tissue hypoxia:
B. Metabolic disorders:
NOTE: Sample collection
Plasma lactate may be falsely elevated if a tourniquet is used.
LACTATE / PYRUVATE RATIO
|
REFERENCE INTERVAL: 6 - 26 (mmol lactate/mmol pyruvate) |
The lactate / pyruvate ratio in plasma is a reflection of the intracellular NADH / NAD ratio. It is of very limited clinical value, except in the diagnosis of specific metabolic disorders in patients with high lactate. An increased ratio is characteristic of tissue hypoxia from any cause, alcohol, and respiratory chain disorders. A normal ratio is can be seen in thiamine deficiency, pyruvate dehydrogenase deficiency, defects of gluconeogenesis, glucose-6-phosphatase deficiency and liver disease.
See also
Refs:
Debray F-G, Mitchell GA, Allard P et al. Diagnostic accuracy of blood lactate-to-pyruvate molar ratio in the differential diagnosis of congenital lactic acidosis. Clin. Chem. 2007;53(5): 916-21)
Emails to chemicalpathologist@adhb.govt.nz will receive priority attention from the on-call chemical pathologist.
If the query concerns a specific patient please include the NHI number in your email.
If email is not a suitable option, please contact the on-call chemical pathologist via Lablink (Auckland City Hospital ext. 22000 or 09-3078995).
Individual chemical pathologists may be contacted but will not be available at all times.
After-hours : contact Lablink (Auckland City Hospital ext. 22000 or 09-3078995) or hospital operator for on duty staff after hours.
Dr Samarina Musaad (Clinical Lead) : SamarinaM@adhb.govt.nz ext. 22402
Dr Cam Kyle: CampbellK@adhb.govt.nz ext 22052
Dr Weldon Chiu: WeldonC@adhb.govt.nz ext. 23427
Dr Campbell Heron: CHeron@adhb.govt.nz ext. 23427
Dr Sakunthala Jayasinghe: Sakunthala@adhb.govt.nz ext. 23427
Lactate/pyruvate ratio:
Test is available only for samples collected at Auckland Hospital.