Specimen Collection
The direct immunofluorescent stain (DFA) is used for the detection of Pneumocystis jirovecii cysts in specimens collected from the respiratory tract.
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Bronchial washings (preferable).
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Induced sputum.
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Sputum. (minimum 4mL)
Samples to be collected in sterile plastic tubes / pottles.
If possible, please try to get the specimen to the laboratory before 1:00pm and notify the laboratory that it is being sent.
Samples stable at ambient temperature for 2 hours.
Samples can be refrigerated for 7 days.
Rejection Criteria
: non-respiratory samples, swabs, tissues and samples in formalin.
If sputa and BAL/Bronchial Washing received, only the BAL/Bronchial Washing is processed.
Turnaround Time:
Routinely performed on weekdays (8:00 am - 2:00 pm)
Three hour turnaround time for urgent specimens.
Notify the laboratory if URGENT
Diagnostic Use and Interpretation
This test is validated for use only on induced sputa and BAL fluid. When the test is performed on expectorated sputa the sensitivity maybe compromised. This needs to be taken into account when interpreting results.
Contact Information
For further information, contact the Mycology laboratory (ext 22710) or:
Dr Sally Roberts
, Microbiologist:
ext 22705
Cellphone
021 674 140
Dr Sharmini Muttaiyah
, Microbiologist:
ext 22700
Cellphone
021 615 892
Dr Mary de Almeida
, Microbiologist:
ext 22700
Cellphone
021 170 9117
Dr Matthew Blakiston
, Microbiologist: contact via Lablink
Result Details
For results, use computer or phone Lablink.
Lablink contact details
Specimen Transport Instructions for Referring Laboratories
Send refrigerated samples at 4 - 7
o
C for testing.