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Mycoplasma genitalium resistance testing PCR

DNA/RNA
Test performed by: LabPLUS Microbiology


Note: For routine screening for Mycoplasma genitalium refer to Mycoplasma genitalium NAAT in the Test Guide.

Specimen Collection

Note: Refer to the Mycoplasma genitalium NAAT for the Aptima collection tubes.

Suitable specimens:

Unsuitable specimens:
  • Swabs in Amies transport media
  • Specimens in Heparin tubes
Females:
  • Vaginal or Endocervical (preferred)
  • Urine specimen
  • Rectal swab
Males:
  • Urine specimen
  • Urethral swab
  • Rectal swab
Aptima collection tubes can be stored and transported at room temperature, all others store and transport at fridge temperature.

See also:

Chlamydia/Gonorrhoea/Trichomonas Nucleic Acid Amplification Test (NAAT)

Mycoplasma genitalium NAAT

STI Specimen Collection Guide


Turnaround Time:

This test is performed in a weekly batch.

Specimens received in the Microbiology laboratory before 1000 hrs on Monday will be processed that day and the interim result available on Wednesday, and the final result on Friday.

Specimens arriving after 1000 hrs on Monday will be held and processed in the following Monday's batch.

Turnaround time excludes weekends and public holidays.

Please discuss with the Clinical Microbiologist if urgent or non-routine specimens.


Assay Method

The assay is performed using in-house PCR and sequencing (DNA) method for Mycoplasma genitalium resistance gene mutation detection.


Diagnostic Use and Interpretation

Mycoplasma genitalium is a sexually transmitted pathogen which causes urethritis in males, pelvic inflammatory disease (PID), acute endometritis, and adnexitis in woman.  Mycoplasma genitalium accounts for as much as 15-20% of all cases of nongonococcal urethritis (NGU).

Specimens that are positive for Mycoplasma genitalium on the Panther (screening test) or referred from other laboratories, will be further tested for resistance genes.

Resistance testing will be performed for the presence of absence of mutations in the 23s rRNA gene as positions A2025 and A2059 ( E. coli numbering), that confers resistance to macrolides and the parC gene that confers resistance to fluoroquinolones.


Contact Information

For further information, contact the Microbiology Department via Lablink  or the Clinical Microbiologist:

Lablink contact details

Dr Sally Roberts , Microbiologist: ext 22705   Cellphone 021 674 140
Dr Sharmini Muttaiyah , Microbiologist: ext 22700   Cellphone 021 615 892
Dr Mary de Almeida , Microbiologist: ext 22700    Cellphone 021 170 9117

Dr Matthew Blakiston , Microbiologist: contact via Lablink
Dr Veronica Playle , Microbiologist: contact via Lablink


Specimen Transport Instructions for Referring Laboratories

Ensure specimens will not leak in transit, otherwise they will be rejected.

Specimens in Aptima tubes are stable and can be transported at room temperature. Other specimens should be stored and transported at fridge temperature.



Last updated at 07:47:05 03/03/2023