The test is only performed Monday - Friday 0800:1700 hrs.
Separate sample for Soluble CD25; cannot be shared with Chemistry.
Internal : Immediately spin and separate plasma to 2 x 1ml aliquots. Hand deliver spun and separated Heparin plasma to Coagulation Lab. Freeze at -20c.
External Laboratories must FREEZE the plasma within 24-hours of collection and send 2 x 1ml Heparin Plasma aliquots to LabPlus frozen.
Uncertainty of Measurement: 20%
| Age Range | Either Sex |
|---|---|
| < 2 y | < 7,887 |
| 2 y - 7 y | < 4,321 |
| >= 7 y | < 2,678 |
Requests for Soluble CD25 must be arranged through LabPlus Haematologist who will advise when results will be available.
Samples were tested using Quantikine R Human CD25/IL-2Ra Immunoassay which is a 3.5 hour solid phase ELISA designed to measure soluble human IL-2Ra in cell cultures ,serum and Heparin plasma. It contains NS0 expressed recombinant human IL-2Ra and antibodies raised against the recombinant factor. Results obtained using natural Human IL-2Ra showed linear curves that were parallel to the standard curves obtained using the Quantikine R kit standard indicating this kit can be used to determine relative mass values for natural human IL-2Ra. This assay employs the quantitative sandwich enzyme immunoassay technique .A monoclonal antibody specific for human IL-2Ra has been pre coated onto a microplate. Standards, samples, and conjugate are pipetted into the wells and any IL-2Ra present is sandwiched by the immobilized antibody and the enzyme linked polyclonal antibody specific for human IL-2Ra.
Elevated serum level of IL2 Receptor alpha ( IL-2Ra ) is important in the diagnosis of HLH (Haemophagocytic Lymphohistiocytosis), which can be used to monitor treatment and to evaluate disease activity.
For further information contact the Haematology laboratory (Ext 22067) or:
| Dr Nicola Eaddy | Ext 22071 | |
| Dr Peter Bradbeer | Ext 22062 | |
| Dr Anna Ruskova | Ext 22137 |
External Laboratories must FREEZE the plasma within 24 hours of collection and send 2 x 1ml Heparin plasma aliquots to Lab Plus Frozen.