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Asialotransferrin
Also known as : [Beta-2 transferrin]



Test performed by: LabPLUS VIM Proteins


Specimen Collection

NOTE: Viscous , heavily bloodstained, or purulent samples are not suitable for analysis.

This assay requires that serum is collected in parallel with the CSF sample.


Sterile Container0.3 mL Sterile Container Fluid (Always Required)

Ear or nasal fluid required.


SST2 mL SST Serum (Preferred)
Plain2 mL Plain Serum
Microsample500 uL Microsample Serum
Micro-SST500 uL Micro-SST Serum
Turnaround Time: Between 1 day and 2 days

Test is performed Monday - Friday.

Samples which arrive in the lab after 1.30 PM on Friday will be processed on the following business day.

If urgent processing is required, please contact the on-call chemical pathologist via Lablink (Auckland City Hospital ext. 22000 or 09-3078995).


Diagnostic Use and Interpretation

Beta-2 transferrin (asialotransferrin or Tau fraction of transferrin) is found in CSF, but not in normal serum or secretion fluids. Detecting this protein in fluid from nose or ear suggests the possibility of CSF leakage, e.g. in patients with skull injuries.

In the method used at Labplus, the isoforms of transferrin are separated by electrophoresis followed by immunochemical staining of the bands. This procedure takes several hours to perform, and is not available after hours or on weekends.

Note that samples with a high protein content (e.g. exudates) may be uninterpretable due to the high concentration of normal transferrin.

References

1.         Cerebrospinal Fluid Leakage:  Agarose gel electrophoresis detection of beta-2 transferrin and Nephelometric quantification of beta-Trace protein.  Clinical chemistry 50, No 12, 2004.

2.         Beta-Trace Protein as a Marker for Cerebrospinal Fluid Rhinorrhea.  Clinical Chemistry 48, No. 6, 2002.

3.         http://www.cdc.gov/ncidod/dhqp/gl_isolation_standard.html


Contact Information

Emails to chemicalpathologist@adhb.govt.nz will receive priority attention from the on-call chemical pathologist.

If the query concerns a specific patient please include the NHI number in your email.

If email is not a suitable option, please contact the on-call chemical pathologist via Lablink (Auckland City Hospital ext. 22000 or 09-3078995).

Individual chemical pathologists may be contacted but will not be available at all times. 

After-hours : contact  Lablink (Auckland City Hospital ext. 22000 or 09-3078995) or hospital operator for on duty staff after hours.


Dr Cam Kyle (Clinical Head): CampbellK@adhb.govt.nz   ext 22052 

Dr Weldon Chiu: WeldonC@adhb.govt.nz   ext. 23427 

Dr Leah Ha: LHa@adhb.govt.nz  ext. 23427

Dr Samarina Musaad: SamarinaM@adhb.govt.nz ext. 22402 

Dr Leo Lam: ChiSingL@adhb.govt.nz  ext 22574



Last updated at 16:57:03 21/03/2023